Journal: Technology in Cancer Research & Treatment
Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy
doi: 10.1177/1533033820944274
Figure Lengend Snippet: BUB1B might be a potential target for immunotherapy in hepatocellular carcinoma (HCC). (A) Association of the BUB1B expression and CD8 + T-cell infiltration in HCC by TISIDB (r = −0.129, P = .013). (B) BUB1B expression level is negatively associated with CD8 + T-cell infiltration level validated in our 72 patients, tested by Spearman test (r = −0.489, P < .001). (C) Representative immunohistochemical staining of BUB1B, β-catenin, and CD8 in HCC. Negative, weak positive, moderate positive, and strong positive expression of BUB1B were shown, respectively. (D) Association of the BUB1B messenger RNA (mRNA) expression and β-catenin mRNA in HCC by GEPIA (r = 0.46, P < .001). (E) BUB1B expression level is positively associated with β-catenin expression level validated in our 72 patients, tested by Spearman test (r = −0.588, P < .001). (F) Overall survival (OS) curves of patients with HCC according to cancer expressed BUB1B levels ( P < .001). (G) Disease-free survival (DFS) curves of patients with HCC according to cancer expressed BUB1B levels ( P = .008).
Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).
Techniques: Expressing, Immunohistochemical staining, Staining